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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
21/02/2014 |
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Actualizado : |
29/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GAIERO, P.; MAZZELLA, C.; VILARÓ, F.; SPERANZA, P.; DE JONG, H. |
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Afiliación : |
PAOLA GAIERO, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; Laboratory of Genetics, Wageningen University, Netherlands; CRISTINA MAZZELLA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; FRANCISCO LUIS VILARO PAREJA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO SPERANZA, Department of Plant Biology, Facultad de Agronomía, Universidad de la República, Uruguay; HANS DE JONG, Laboratory of Genetics, Wageningen University, Netherlands. |
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Título : |
Pairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Euphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS. |
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ISSN : |
0014-2336 |
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DOI : |
10.1007/s10681-017-1922-4 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017. |
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Contenido : |
ABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato.
© 2017, The Author(s). MenosABSTRACT.
Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental spe... Presentar Todo |
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Palabras claves : |
BAC FISH; GISH; Introgressive hybridisation; Pairing behaviour; Potato wild relatives. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
https://link.springer.com/content/pdf/10.1007%2Fs10681-017-1922-4.pdf
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Marc : |
LEADER 02814naa a2200265 a 4500
001 1012827
005 2019-10-29
008 2017 bl uuuu u00u1 u #d
022 $a0014-2336
024 7 $a10.1007/s10681-017-1922-4$2DOI
100 1 $aGAIERO, P.
245 $aPairing analysis and in situ Hybridisation reveal autopolyploid-like behaviour in Solanum commersonii × S. tuberosum (potato) interspecific hybrids.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: 29 December 2016 / Accepted: 29 May 2017 / Published online: 14 June 2017.
520 $aABSTRACT. Wild potato relatives are rich sources of desirable traits for introgressive hybridisation into cultivated potato. One of them, Solanum commersonii (2n = 2x = 24, 1EBN, endosperm balance number), is an important species belonging to the potato tertiary genepool. It can be used in potato breeding through bridge crosses and 2n gamete production. Triploid F1 hybrids between S. commersonii (through spontaneous 2n egg formation) and diploid 2EBN Solanum tuberosum Group Phureja were crossed with S. tuberosum Group Tuberosum, resulting in successive backcross 1, 2 and 3 progenies. The main aim of this study was to determine if there are any barriers to homoeologous pairing and recombination in the allotriploid (S. commersonii × potato) hybrids and their backcrosses, and so to predict if S. commersonii chromosomes can be transmitted to the next generation and introgressed into their recipient potato chromosomes. Microscopic observations of spread pollen mother cells suggested no preferential pairing in the triploid hybrids, while chromosome transmission and segregation in further meiotic stages were fairly balanced. Fluorescent in situ hybridisation with BAC probes (BAC FISH) was used to obtain markers to trace the meiotic behaviour of specific chromosome pairs. Moreover, genomic in situ hybridisation (GISH) demonstrated no obvious differences in fluorescence signals between the homoeologues suggesting that repetitive sequences did not diverge much between the parental species. As a consequence, we were not able to trace the course of the S. commersonii chromosomes in the successive introgressive hybridisation backcross generations. Our results strongly point at a high genomic similarity between the homoeologous chromosomes promising high suitability of S. commersonii in introgressive hybridisation breeding of potato. © 2017, The Author(s).
653 $aBAC FISH
653 $aGISH
653 $aIntrogressive hybridisation
653 $aPairing behaviour
653 $aPotato wild relatives
700 1 $aMAZZELLA, C.
700 1 $aVILARÓ, F.
700 1 $aSPERANZA, P.
700 1 $aDE JONG, H.
773 $tEuphytica, 1 July 2017, Volume 213, Issue 7, Article number 137. OPEN ACCESS.
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
04/09/2019 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DI BELLA, C.E.; GARCÍA-PARISI, P.A.; LATTANZI, F.; DRUILLE, M.; SCHNYDER, H.; GRIMOLDI, A.A. |
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Afiliación : |
CARLA E. DI BELLA, IFEVA, Universidad de Buenos Aires, CONICET, Facultad de Agronomía Buenos Aires./Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Cátedra de Forrajicultura Buenos Aires Argentina.; PABLO A. GARCÍA-PARISI, IFEVA, Universidad de Buenos Aires, CONICET, Facultad de Agronomía Buenos Aires./Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Cátedra de Forrajicultura Buenos Aires Argentina.; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Technische Universität MünchenFreising-Weihenstephan Germany.; MAGDALENA DRUILLE, Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Cátedra de Forrajicultura, Buenos Aires Argentina.; HANS SCHNYDER, Technische Universität MünchenFreising-Weihenstephan Germany.; AGUSTÍN A. GRIMOLDI, IFEVA, Universidad de Buenos Aires, CONICET, Facultad de Agronomía Buenos Aires./Universidad de Buenos Aires, Facultad de Agronomía, Departamento de Producción Animal, Cátedra de Forrajicultura Buenos Aires Argentina. |
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Título : |
Grass to legume facilitation in saline-sodic steppes: influence of vegetation seasonality and root symbionts. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Plant and soil, 2019. |
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DOI : |
10.1007/s11104-019-04247-y |
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Idioma : |
Inglés |
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Notas : |
Article hisotory: Received: 18 February 2019 /Accepted: 1 August 2019. |
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Contenido : |
Abstract: Aim Identify key factors driving legume seedlings performance in saline-sodic soils. Methods Five plots were established in paired subhumid steppes with saline-sodic soils dominated by Distichlis spicata or Panicum coloratum. In each plot, Lotus tenuis was sown in autumn and individual plants were collected close to nurse plants (dominant species), and in open areas, at the end of the cold and warm seasons. Carbon and nutrient acquisition (through C and O isotopic composition and N and P content measurements) and plant symbiotic functioning (through N
fixation by rhizobia and mycorrhizal colonization measurements) were determined. Results Biomass of legume grown close to a nurse plant was always higher than in open areas. This increase was higher close to P. coloratum and to D.spicatainthecoldandwarmseasons,respectively. In both cases, L. tenuis improved its nutrient acquisition and symbiosis functioning. N-acquisition and rhizobia efficiency increased in the most facilitated L.tenuis plants in the cold season while P-acquisition was greater in the warm season. Conclusions Grass-to-legume facilitation in sub-humid steppes with saline-sodic soils was detected in cold and warm seasons, differing between steppes in relationship with the vegetation growing rate and the establishment and functioning of legume-rhizobia-mycorrhiza symbioses. |
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Palabras claves : |
DUAL ISOTOPE APPROACH; HALOMORPHIC SOILS; LOTUS TENUIS; MYCORRHIZA; N FIXATION; N ISOTOPIC COMPOSITION. |
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Thesagro : |
LEGUMINOSAS. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02255naa a2200289 a 4500
001 1060138
005 2019-10-15
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1007/s11104-019-04247-y$2DOI
100 1 $aDI BELLA, C.E.
245 $aGrass to legume facilitation in saline-sodic steppes$binfluence of vegetation seasonality and root symbionts.$h[electronic resource]
260 $c2019
500 $aArticle hisotory: Received: 18 February 2019 /Accepted: 1 August 2019.
520 $aAbstract: Aim Identify key factors driving legume seedlings performance in saline-sodic soils. Methods Five plots were established in paired subhumid steppes with saline-sodic soils dominated by Distichlis spicata or Panicum coloratum. In each plot, Lotus tenuis was sown in autumn and individual plants were collected close to nurse plants (dominant species), and in open areas, at the end of the cold and warm seasons. Carbon and nutrient acquisition (through C and O isotopic composition and N and P content measurements) and plant symbiotic functioning (through N fixation by rhizobia and mycorrhizal colonization measurements) were determined. Results Biomass of legume grown close to a nurse plant was always higher than in open areas. This increase was higher close to P. coloratum and to D.spicatainthecoldandwarmseasons,respectively. In both cases, L. tenuis improved its nutrient acquisition and symbiosis functioning. N-acquisition and rhizobia efficiency increased in the most facilitated L.tenuis plants in the cold season while P-acquisition was greater in the warm season. Conclusions Grass-to-legume facilitation in sub-humid steppes with saline-sodic soils was detected in cold and warm seasons, differing between steppes in relationship with the vegetation growing rate and the establishment and functioning of legume-rhizobia-mycorrhiza symbioses.
650 $aLEGUMINOSAS
653 $aDUAL ISOTOPE APPROACH
653 $aHALOMORPHIC SOILS
653 $aLOTUS TENUIS
653 $aMYCORRHIZA
653 $aN FIXATION
653 $aN ISOTOPIC COMPOSITION
700 1 $aGARCÍA-PARISI, P.A.
700 1 $aLATTANZI, F.
700 1 $aDRUILLE, M.
700 1 $aSCHNYDER, H.
700 1 $aGRIMOLDI, A.A.
773 $tPlant and soil, 2019.
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